RealHelix™ qRT-PCR Kit [Green] 은 green fluorescent dye가 적용된 one-step quantitative RT-PCR 제품입니다. 본 제품의 enzyme mix는 reverse transcriptase, chemically-modified hot-start Taq polymerase (Hot-Taq polymerase) 및 RNase inhibitor가 최적화된 조건으로 혼합되어 있어서 높은 특이도(specificity)와 민감도(sensitivity)를 가지고 있습니다. Buffer mix는 반응에 최적화된 buffer components와 함께 Mg²⁺, dNTPs, green fluorescent dye 및 stabilizing agent가 혼합되어 있습니다.

Figure. Comparison of RealHelix™ qRT-PCR Kit [Green] and qRT-PCR Kit [Intercalator type] (the previous version).

10-fold serial dilutions of the human total RNA (1, 0.1, and 0.01 ng) from whole blood were analyzed using a human beta actin gene-specific primer set. RealHelix™ qRT-PCR Kit [Green] shows more reliable amplifications than our previous version. Reactions were run on a real-time PCR instrument, CFX96 (Bio-Rad, USA).

Storage

• Store below -20 ℃
• Should be stored in a dark place.

Shelf life

• 12 months